Selectoprobe™ polyHRP-Streptavidin Conjugate
$590.00 – $5,600.00
Catalog No.: SP-F-0158
Description: Immunoassays (such as histochemistry, ELISAs and Western blotting) are important in clinical diagnosis and experimental protein analysis. These indirect analytical strategies primarily rely on the binding of an antigen by a specific antibody (primary antibody), followed by the detection of primary antibody by an enzyme or fluorescently labelled secondary antibody, allowing the semi-quantitative detection of a protein of interest. Histochemistry, ELISAs and Western blotting have been the common choices for protein validation studies for the past several decades. Technical advancements and modifications are continuously being developed to enhance the detection sensitivity of these procedures. Among them, streptavidin-containing poly-horseradish peroxidase (PolyHRP) based detection strategies have been shown to improve signals in a variety of immunoassays. The commercially available streptavidin and antibodies conjugated with many HRPs (PolyHRPs) have been widely used to enhance the detection sensitivity in immunoassays.
Selectoprobe™ polyHRP-Streptavidin Conjugate is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays. Due to the extremely high sensitivity of Selectoprobe™ polyHRP-Streptavidin Conjugate a blocking procedure may been needed to eliminate the interference caused by the endogenous biotinylated proteins.
Storage: Refrigerated (2-8 °C); Minimize light exposure
Reference:
Li, Dongyang and Cui, Yongliang and Morisseau, Christophe and Gee, Shirley J and Bever, Candace S and Liu, Xiangjiang and Wu, Jian and Hammock, Bruce D and Ying, Yibin, Nanobody Based Immunoassay for Human Soluble Epoxide Hydrolase Detection Using Polymeric Horseradish Peroxidase (PolyHRP) for Signal Enhancement: The Rediscovery of PolyHRP, Analytical chemistry (2017): 6248-6256
Mishra, Manish and Tiwari, Shuchita and Gunaseelan, Anita and Li, Dongyang and Hammock, Bruce D and Gomes, Aldrin V, Improving the sensitivity of traditional Western blotting via Streptavidin containing Poly-horseradish peroxidase (PolyHRP). Electrophoresis (2019): 1731-1739
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