Selectoprobe™ Eu donkey anti-goat IgG (H+L) (Cross Adsorbed)

$598.00$145,600.00

Catalog No.: SP-F-0154

Description: The Selectoprobe™ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobe™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules.

This lyophilized Selectoprobe™ Eu donkey anti-goat IgG (H+L) conjugate (Cross Adsorbed) is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.

Storage: Freeze (< -15 °C); Minimize light exposure

Correction Factor (260 nm)
0.911
Correction Factor (280 nm)
0.777
Extinction coefficient (cm -1 M -1)
21000
Excitation (nm)
298
Emission (nm)
617

Reference:

Gaborit N, Larbouret C, Vallaghe J, Peyrusson F, Bascoul-Mollevi C, Crapez E, Azria D, Chardes T, Poul MA, Mathis G, Bazin H, Pelegrin A., Time-resolved fluorescence resonance energy transfer (TR-FRET) to analyze the disruption of EGFR/HER2 dimers: a new method to evaluate the efficiency of targeted therapy using monoclonal antibodies, J Biol Chem, 2011, 11337