Fluorophores, also known as fluorescent dyes or fluorescent probes, are molecules capable of absorbing light at specific wavelengths and then emitting light at longer wavelengths. Fluorophores are versatile tools with widespread applications in biomedical research, diagnostics, environmental monitoring, and materials science. They are frequently covalently linked to macromolecules, functioning as markers, dyes, tags, or reporters for molecules such as antibodies, peptides, or nucleic acids. Fluorophores are extensively employed in analytical methods, including fluorescent imaging and spectroscopy, for staining tissues, cells, or materials.
Historically, fluorescein and its amine-reactive derivative, fluorescein isothiocyanate (FITC), have been widely utilized, initially in antibody labeling and later expanding to nucleic acids with carboxyfluorescein. Other commonly used fluorophores include derivatives of rhodamine (e.g., TRITC), coumarin, and cyanine. However, newer generations of fluorophores, often offer enhanced performance characteristics such as increased photostability, brightness, or reduced pH sensitivity compared to traditional dyes with similar excitation and emission properties.
Description: The Selectoprobe™ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobe™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules.
This lyophilized Selectoprobe™ Eu goat anti-mouse IgG (H+L) conjugate is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.
Description: The Selectoprobe™ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobe™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. This lyophilized Selectoprobe™ Eu goat anti-mouse IgG (H+L) conjugate (Cross Adsorbed Antibody) is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.
Description: The Selectoprobe™ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobe™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules.
This lyophilized Selectoprobe™ Eu goat anti-rabbit IgG (H+L) conjugate is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.
Description: The Selectoprobe™ Eu probe enable time-resolved fluorometry (TRF) for the assays that require high sensitivity, showing large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, Selectoprobe™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. This lyophilized Selectoprobe™ Eu goat anti-rabbit IgG (H+L) conjugate (Cross Adsorbed Antibody) is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with primary antibodies.
Description: This lyophilized Selectoprobe™ Eu-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with Selectoprobe™ Eu covalently attached (as the time-resolved red fluorescent europium label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using TR-FRET platform. A variety of the complementary biotinylated reagents are available from Selectoprobe™.
Description: The Europium cryptate labeling reagent is specifically designed for biomolecule labeling, enabling the rapid and efficient development of time-resolved fluorescence resonance energy transfer (TR-FRET) assays. It facilitates quick and easy labeling of peptides, proteins, and oligonucleotides.
The Selectoprobe™ Eu DBCO (europium complex) can be readily clicked to molecules containing an azido group under copper-free conditions.
Compared to the other compounds, our cryptate Eu probes have extremely high stability, high emission yield and ability to selectively label biomolecules. Moreover, our Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.
$880.00 – $69,880.00Select options
This product has multiple variants. The options may be chosen on the product page
Description: The Europium cryptate labeling reagent is specifically designed for biomolecule labeling, enabling the rapid and efficient development of time-resolved fluorescence resonance energy transfer (TR-FRET) assays. It facilitates quick and easy labeling of peptides, proteins, and oligonucleotides.
The N-hydroxysuccinimide (NHS)-activated Europium cryptate reacts with the primary amines on biomolecules, allowing for simplified and efficient conjugation.
Compared to the other compounds, our Selectoprobe™ Cryptate Eu probes have extremely high stability, high emission yield and ability to selectively label biomolecules. Moreover, our Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies.
$500.00 – $68,571.00Select options
This product has multiple variants. The options may be chosen on the product page
Selectoprobe™ polyHRP-Donkey Anti-Goat IgG Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The polyHRP-Donkey Anti-Goat is purified to remove unconjugated goat anti-mouse IgG molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. Selectoprobe™ polyHRP-Donkey Anti-Goat IgG is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.
Description: Goat anti-mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Selectoprobe™ polyHRP-Goat Anti-Mouse IgG Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The goat anti-mouse IgG poly-HRP conjugate is purified to remove unconjugated goat anti-mouse IgG molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. Selectoprobe™ polyHRP-Goat Anti-Mouse IgG is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.
Description: Goat anti-rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Selectoprobe™ polyHRP-Goat Anti-Rabbit IgG Conjugate is designed to deliver the highest sensitivity and low background in immunoassays where sample volume is limited or when the target molecule is present at low levels. The goat anti-rabbit IgG poly-HRP conjugate is purified to remove unconjugated goat anti-mouse IgG molecules that competes for binding sites with its HRP-conjugates. In addition, the conjugate is devoid of unconjugated HRP that can cause background signal. Selectoprobe™ polyHRP-Goat Anti-Rabbit IgG is compatible with chromogenic, fluorogenic and chemiluminescent HRP substrates used in ELISA, Western blotting, immunohistochemistry (IHC) and nucleic acid hybridization assays.
